AAV is a recombinant DNA molecule that is used as a delivery vehicle for gene therapy. It is composed of two DNA strands, each containing a single gene of interest. The vector can be inserted into the cells using standard transfection methods such as electroporation and lipofection. Once in the cell, the AAV vector can express the gene of interest and carry out its desired function. You can also learn more about AAV transfection methods by visiting at: www.bosterbio.com/services/aav-packaging-service.
There are different ways to use AAV to deliver genes into cells. One method is to use AAV vectors that contain both the gene of interest and a promoter to drive expression. This approach is often used when the gene of interest is transcriptionally inactive or when it needs to be expressed at high levels.
Transfection with an AAV vector is a delicate process that requires careful consideration. Many factors can affect transfection, including the virus particle size, pH, and other chemicals present in the transfection solution. In addition, it is important to select an appropriate transfection method and provide adequate media conditions for the cells.
If you're ever confused about what an AAV expression, purification and transfection (AET) experiment is or how it works, this article should help clear things up. Essentially, an AET experiment is a way to study gene expression in cells by using adeno-associated viruses (AAVs). These viruses are specially engineered so that they can enter the cell and replicate without causing harm.